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KMID : 0382320060260030063
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Environmental Mutagens and Carcinogens
2006 Volume.26 No. 3 p.63 ~ p.68
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Chromosome Aberration Test of Water Extract of Decaffeined Green Tea using Supercritical Carbon Dioxide with Mammalian Cell Line
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Koo Yun-Chang
Kim Kwang-Ok
Chung Young-Sin
Park Byung-Gyu
Lee Hyun-Sun
Lee Sun-Joo
Lee Kwang-Won
Kim Eun-Jin
Kim Kyoung-Hoen
Kim Young-Suk
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Abstract
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There are 10~30% polyphenol and 2~4% caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study. We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ㎍/㎖ and compared with the negative controls, there were no significant (P ＞ 0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.
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KEYWORD
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Decaffeined green tea, Supercritical carbon dioxide, Chromosome aberration test
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